Performance of multiplex celiac antibody test in the diagnosis of pediatric celiac disease (CD)

For one study, researchers sought to determine how an automated celiac disease (CD) serology analyzer (Bioplex2200) performed in real-life situations and find a link between TGA levels and intestinal biopsies in children. Patients with both anti-tissue transglutaminase (TGA) antibody serologic testing and duodenal biopsies were included in a retrospective evaluation conducted at 2 pediatric gastroenterology facilities between November 1, 2018 and April 1, 2020. Patient demographics, medical history, TGA levels, and biopsy results were among the information retrieved. A total of 538 children were assessed, including 256 with a positive TGA (68.4% girls, median age of 6.4 years) and 282 with a negative TGA (53.9% girls, median age of 13. Four years). In 219 patients who had a positive TGA, intestinal biopsies confirmed CD (85.5%). Overall, 14.5% of the cohort had positive serology with normal histology, with 52%, 21.6%, 21.1% and 4.2% in TGA ranges of 1-3 ULN, 3 to 5 LSN, 5 to 10 LSN and more than 10 LSN. times LSN, respectively, Pperformance was lower in this subgroup, with an AUC of 0.737 (95% CI 0.834-0.839). In real situations, the Multiplex TGA test showed perfect diagnostic accuracy. Adding EMA to patients with a positive TGA did not improve the diagnostic efficiency of the test. False positive rates varied between TGA ranges, but were low when the TGA exceeded 10 times the ULN.

Source:journals.lww.com/jpgn/Abstract/2022/04000/Real_life_Performance_of_Multiplex_Celiac_Antibody.13.aspx